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TIRF, total internal reflection fluorescenceĬHANNELS-ESSENTIAL FOR NEURONAL EXCITABILITY.FRET, fluorescent resonance energy transfer.FRAP, fluorescence recovery after photobleaching.BNFC, benign neonatal familial convulsions.The characterisation of the membrane channels by techniques that resolve their morphological and physical properties and dynamics in space and time in the nano range is termed nanoscopy. These are the patch clamp technique, which was derived from the conventional voltage clamp method and is currently being developed for automated and high throughput measurements and fluorescence and nano-techniques, which were originally applied to non-biological surfaces and are only recently being used to study cell membranes and their proteins, especially in combination with the patch clamp technique. This review deals with the techniques that make the study of structure and function of single channel molecules in living cells possible. 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